Plasmid

Part:BBa_M50052:Design

Designed by: Nicholas M Kuntz   Group: Stanford BIOE44 - S11   (2016-12-11)


alkB1 + alkG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
    Illegal BglII site found at 976
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1573
    Illegal SapI.rc site found at 643


Design Notes

Refer to individual design considerations for BBa_M50005 and BBa_M50004


Source

Sourced from genome of A. borkumensis bacterium

References